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Kinetic profiles of photocurrents.

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posted on 23.09.2010, 02:37 authored by Lei Wen, Hongxia Wang, Saki Tanimoto, Ryo Egawa, Yoshiya Matsuzaka, Hajime Mushiake, Toru Ishizuka, Hiromu Yawo

A–C. Photocurrent traces of ChR1-fg2 (ChRGR, A), ChR1 (B) and ChR2 (C) evoked by green LED with various strengths (505±15 nm, 1 s pulse, 0.064–0.77 mWmm−2). All experiments were done at 34°C. Each photocurrent was normalized to the peak value evoked by the maximal level. D–F. Parameters of photocurrent kinetics. All experiments were done at 34°C. (D) The apparent ON time constant (τON)-light power density (L) relationships. Each relationship was fitted to a function, τON = (a+bL)−1 [28]. (E) The desensitization-light power density relationships. Each relationship was fitted to a Michaelis-Menten-type kinetics [22]. (F) The effective OFF time constant (τOFF)-light power density relationships. Each relationship was fitted to a first order function. G. Typical traces of ChRGR photocurrents in response to double light pulse stimulation by green LED (505±15 nm, 1 s pulse, 0.77 mWmm−2). H. The recovery time course of the peak current (Ipeak). I. The recovery time course of the steady-state current (Iss). J. The recovery time course of the desensitizing component. The process was fitted to a single exponential relationship (time constant, 1.3 s).

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