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Kaiso suppresses β-catenin mRNA expression in cell lines that have not been treated with 5-Aza-CdR.

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posted on 2014-02-03, 08:03 authored by Yang Liu, Qian-Ze Dong, Si Wang, Hong-Tao Xu, Yuan Miao, Liang Wang, En-Hua Wang

Lung cancer cell lines SPC (A) and LTE (E) were transfected with Kaiso cDNA plasmid. The effect of transfection at different time points was identified by Western blot. The Kaiso-specific band appears at 97 kDa. Statistical analysis of SPC (B) or LTE (F) by t-test showed a significant increase Kaiso expression in the cells transfected with Kaiso cDNA plasmid compared with the control cells (B: P = 0.003 for 24 h, P = 0.005 for 48 h, and P<0.001 for 96 h, respectively; F: P = 0.065 for 24 h, P = 0.007 for 48 h; P = 0.009 for 96 h, respectively). Analysis of β-catenin mRNA expression in SPC following treatment of using RT-PCR (C) and Real-Time PCR by ANOVA (D) showed that treatment with 5-Aza-CdR demethylation reagent (7 µmol/L) for 48 h resulted in significant upregulation (P = 0.007), whereas high Kaiso expression significantly down-regulated β-catenin mRNA expression (P = 0.004). The expression of β-catenin mRNA expression in cells treated with 5-Aza-CdR reagent did not change significantly in the presence of high Kaiso expression (P = 0.062). Similar effects were observed in the LTE cell line (G and H; H: P = 0.003, P = 0.001, and P = 0.055 accordingly).