KSHV infection results in upregulation of AID in Primary Human Tonsillar B cells.
(A) Primary tonsillar cells were infected with KSHV by co-culture with reactivated iSLK.219 for the indicated time. Percent infection of B cells was assessed via flow cytometry by gating on CD19+ cells and quantifying infected, GFP+ cells (right rectangular gate). (B) Intracellular AID expression was assessed in infected, GFP+ B cells (red) and uninfected, GFP− B cells (filled gray) using flow cytometry. Black histogram represents unstained control. (C) Tonsillar cells were infected by co-culture with reactivated iSLK.219 cells for 2 days. CD19+ population was enriched (see figure S1) and subsequently sorted for infected, GFP+ and uninfected, GFP− cells. The level of AID transcript was measured by qRT-PCR. Shown are mean relative values ± SD from 4 independent patients (*p<.01 by two-tailed, paired student t-test). (D) Tonsillar cells were transiently transfected with vFLIP (red) or vector control (gray) plasmids. 48 hrs post transfection CD19+, GFP+ (transfection marker) cells were analyzed for intracellular AID by flow cytometry. Black histogram represents unstained control.