KEGG metabolic pathways containing chokepoint reactions for which a drug-like compound showed activity.

Chokepoint reactions (chokepoint enzymes, with its substrate and product) are highlighted in red. A. Pathway Maps for Steroid Hormone Biosynthesis (ko00140). Chokepoint enzyme 1.3.99.5 is involved in a chokepoint reaction in this pathway. 5α-Androstane-3,17-dione (C00674) and 5α-Dihydro-testosterone (C03917) are chokepoint compounds. Azelic acid (drug-like compound 7, DB00548) targets 3-oxo-5-alpha-steroid 4-dehydrogenase (EC 1.3.99.5) as well as other targets. Exposing C. elegans to azelic acid led to a movement-impaired phenotype. B. Pathway Maps for Tryptophan Metabolism (ko00380). Enzyme 4.1.1.28 is involved in a chokepoint reaction in this pathway. 5-Hydroxykynurenamine (C05638), Serotonin (C00780), and Trypamine (C00398) are chokepoint compounds. Carbidopa (drug-like compound 2, DB00190) targets DOPA decarboxylase (EC 4.1.1.28). Exposing C. elegans to carbidopa produced a movement-impaired phenotype.