posted on 2014-02-28, 04:18authored byChunhong Feng, Kai He, Chunyan Zhang, Song Su, Bo Li, Yuxiao Li, Chun-Yan Duan, Shaokun Chen, Run Chen, Youping Liu, Hong Li, Mei Wei, Xianming Xia, Rongyang Dai
(A) After treated with SP600125 (SP, 20 µM) for indicated time periods, GRP78 was analyzed using western blot in QBC939, RBE and HCCC-9810 cells. (B) After transfected with siJNK for 60 h, GRP78 was analyzed using western blot in QBC939, RBE and HCCC-9810 cells. (C) After treated with salubrinal (Sal, 25 µM) for 30 h with or without SP600125 (SP, 20 µM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells. (D) After treated with tunicamycin (Tun, 2.0 µg/ml) for 24 h with or without SP600125 (SP, 20 µM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells.