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Involvement of Raf-MEK-ERK pathway in AGS-IV treated PC12 cells.

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posted on 11.05.2015 by Rongcai Yue, Xia Li, Bingyang Chen, Jing Zhao, Weiwei He, Hu Yuan, Xing Yuan, Na Gao, Guozhen Wu, Huizi Jin, Lei Shan, Weidong Zhang

PC12 cells were treated with different concentrations (25, 50 and 100 μM) of AGS-IV and then co-incubated with or without 5 mM glutamate (Glu) for 24 h. Data shown are the results of three different experiments and are represented as the relative densities of protein bands normalized to β-actin. Results are presented as means ± SEM of three assays. ** Significant difference compared with GLUs by one-way ANOVA analysis of variance with Tukey’s HSD post hoc test.