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Intracellular synthesis of pro- and anti-inflammatory cytokines in native CD14+ monocytes.

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posted on 28.01.2016, 12:38 authored by Kirsten Glaser, Markus Fehrholz, Tore Curstedt, Steffen Kunzmann, Christian P. Speer

Native CD14+ cells (n = 4) were incubated with LPS 100ng/ml, 100μg/ml Curosurf, 100μg/ml POPG, 100μg/ml PLs, 100μg/ml PLs+SP-B, 100μg/ml PLs+SP-C and 100μg/ml CHF5633 for 14h. Unstimulated CD14+ cells served as negative control, LPS-stimulated monocytes served as positive control. Intracellular concentrations of TNF-α (A), IL-1β (B), IL-8 (C) and IL-10 protein (D) are given as mean ± SD (*p < 0.05, **p < 0.01).