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Intracellular signalling of plasma-induced ROS.

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posted on 15.08.2014 by Paola Brun, Surajit Pathak, Ignazio Castagliuolo, Giorgio Palù, Matteo Zuin, Roberto Cavazzana, Emilio Martines

Representative Western blot analysis of PPAR-γ in protein extracts from HSCs and ISEMFs treated with plasma and incubated for 4 hrs. As indicated, cells were pre-treated with NAC. Similar results were obtained in three independent experiments. β-actin was used as a loading control (panel A). Cells were treated for 30 min with 50 µM PPAR-γ receptor antagonist T0070907, exposed to plasma, and then incubated under standard conditions for 24 hrs. IL-6 (panel B) and IL-1β (panel C) were assessed in the conditioned medium by ELISA. Data are reported as mean±SE of results collected in nine independent experiments, each performed in triplicate. * denotes P<0.05 vs non treated cells.