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Intestinal specific expression of xbp-1 is sufficient to rescue sensitivity to the PFT.

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posted on 2008-10-10, 02:40 authored by Larry J. Bischof, Cheng-Yuan Kao, Ferdinand C. O. Los, Manuel R. Gonzalez, Zhouxin Shen, Steven P. Briggs, F. Gisou van der Goot, Raffi V. Aroian

Sensitivity to Cry5B was compared among wild-type N2, xbp-1(zc12), xbp-1(zc12) transformed with app-1::GFP, and xbp-1(zc12) transformed with app-1::xbp-1 animals using a plate feeding assay. (A) The health of the worms (details in Materials and Methods) was evaluated after 72 hours on 25% Cry5B-expressing E. coli. Three and six independent lines of app-1::GFP and app-1::xbp-1 were used, respectively. Data are mean and standard deviation of three experiments. (B) Images comparing the health of wild-type N2, xbp-1(zc12), xbp-1(zc-12) app-1::GFP, and xbp-1(zc-12) app-1::xbp-1 animals on 25% Cry5B plates for 72 h. Scale bar is 0.2 mm.