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Interaction of VEGFR-2 (A) or HER-2 (B) or EGFR (C) or 6×his-tag (D) with MdOS by surface plasmon resonance analysis.

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posted on 21.02.2013 by Jingui Ma, Xianliang Xin, Linghua Meng, Linjiang Tong, Liping Lin, Meiyu Geng, Jian Ding

VEGFR2 was injected at the concentrations of 2.56, 0.64, 0.32, 0.16 and 0.08 µM (from top to bottom); HER-2 was injected at the concentrations of 0.53, 0.26, 0.13, 0.07 and 0.03 µM (from top to bottom); EGFR was injected at the concentrations of 6, 3, 1.5, 0.75, 0.38, 0.19 and 0.09 µM (from top to bottom); 6×his-tag was injected at the concentrations of 100, 10, 1, 0.1, 0.01 µM. Sensorgram responses at equilibrium were plotted against each concentration of compounds and the equilibrium dissociation constant (KD) of the binding system was calculated using BIAeval software 3.1.

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