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Interaction of SP-R210 with innate immune receptors.

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posted on 12.05.2015, 02:54 by Linlin Yang, Marykate Carrillo, Yuchieh M. Wu, Susan L. DiAngelo, Patricia Silveyra, Todd M. Umstead, E. Scott Halstead, Michael L. Davies, Sanmei Hu, Joanna Floros, Francis X. McCormack, Neil D. Christensen, Zissis C. Chroneos

Immunoprecipitation experiments were carried out using 5 mg/mL (A and B) or 2.5 mg/mL (C) cell extracts from control and SP-R210L(DN) macrophages with polyclonal anti-SP-R210 (A) or monoclonal CD11b (B). To assess the effect of LPS treatment, immunoprecipitation reactions were carried out 10, 30, 60, and 120 min after treatment with 100 ng/mL LPS (C). Co-precipitated proteins were separated on SDS-PAGE gels and blotted with indicated antibodies. Extracts immunoprecipitated with monoclonal anti-SP-R210 were re-probed with polyclonal SP-R210 antibodies. A monoclonal IgG1 against an unrelated viral antigen served as control (C). Results shown are representative of 2–4 independent experiments.