Interaction between MyoD and Id2 reassembles Och1p and reverses och1Δ phenotypes.
(A) The temperature and Congo red sensitivity of och1Δ yeast can be rescued by co-expression of the interacting LOC-MyoD and Id2-CAT pair. och1Δ yeast were transformed with the indicated plasmids and grown on CSM-Leu-Ura agar plates at 30° in the presence of Congo red or at 37°C in the absence of Congo red. Each row shows ten-fold serial dilutions of the indicated strain. (B) Yeast strains were incubated with Alexa488-WGA and fluorescence was measured by flow cytometry. Decreased WGA binding is caused by co-expressing interacting LOC and CAT fusions (LOC-MyoD and Id2-CAT) in the och1Δ strain.