Insertion mutant but active urease can affect bacterial colonisation.
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a) Persistence over 15 months expressed as percentage of colonised mice at each time point (n = 5–15). b) Colonisation level of mice infected with H. pylori expressing mutant urease after 15 months (n = 5; median displayed). c) Persistence of H. pylori expressing mutant urease as indicated by anti-H. pylori IgG levels (n = 12–20; median displayed). Strains were recombinant at either sites 1, 4 or 8 in urease. d) Comparison of anti-H. pylori IgG and anti-UreB IgG levels resulting from colonisation of mice for 3 months with X47 expressing wild-type urease, X47 (wt), or urease mutant at site 8, X47 (site8), (n = 10–20; median displayed).