Inhibition of the secretory pathway does not inhibit Y. pestis intracellular survival.
RAW264.7 macrophages were treated with 0, 0.2 or 10 μM BFA prior to infection with Y. pestis CO92 pCD1(-) LuxPtolC (MOI 10). Extracellular bacteria were killed with gentamicin and intracellular bacterial numbers were monitored at (A) 2 h and (B) 10 h post-infection by bioluminescence. To determine if BFA treatment impacted the ability of Y. pestis to inhibit YCV acidification, macrophages treated with 10 μM BFA were incubated with Lysotracker Red DND-99 prior to infection with live or paraformaldehyde killed Y. pestis CO92 pCD1(-) pGEN222 (MOI 3). Bacterial Colocalization with Lysotracker Red DND-99 was determined by confocal microscopy at (C) 20 and (D) 80 min post-infection. ns = not significant.