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Inhibition of nidovirus RTC activity by Zn2+ can be reversed by chelation.

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posted on 2010-11-04, 00:14 authored by Aartjan J. W. te Velthuis, Sjoerd H. E. van den Worm, Amy C. Sims, Ralph S. Baric, Eric J. Snijder, Martijn J. van Hemert

(A) Schematic representation of the in vitro assays with isolated RTCs, which were initiated with [α-32P]CTP, either in the absence (sample 1 and 2) or presence of 500 µM Zn2+. After a 30-min incubation at 30°C, both the untreated and Zn2+-treated samples were split into two aliquots, and 1 mM of the Zn2+ chelator MgEDTA was added to samples 2 and 4. All reactions were subsequently incubated for another 70 min before termination. (B) Analysis of RNA products synthesized in assays with EAV RTCs. Numbers above the lanes refer to the sample numbers described under (A). (C) In vitro activity assay with SARS-CoV RTCs.