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Inhibition of adherence with samples from chinchillas immunized with His-tagged MhaB protein.

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posted on 02.07.2013 by Teresa L. Shaffer, Rachel Balder, Sean W. Buskirk, Robert J. Hogan, Eric R. Lafontaine

The WT M. catarrhalis strains O35E, O12E, and McGHS1 were preincubated for 30 min at 37°C with pooled samples from chinchillas sham-vaccinated with PBS (black bars) or with pooled samples from chinchillas immunized with His-tagged MhaB at dilutions of 1∶50, 1∶100, 1∶200 and/or 1∶2000. These bacteria were then used to perform adherence assays. The adherence of M. catarrhalis preincubated with samples from chinchillas vaccinated with PBS was set at 100%. The adherence of M. catarrhalis preincubated with samples from chinchillas immunized with His-tagged MhaB is expressed as the percentage (±standard error) of that of M. catarrhalis preincubated with samples from chinchillas vaccinated with PBS. Assays were performed in triplicate on three separate occasions. The asterisks indicate that the reduction in adherence is statistically significant (P values <0.05, paired t test). Post-boost samples taken on Day 44 of vaccination experiments (see Fig. 3) were pooled and used in these assays.

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