Inhibition of PyAeADHII at 70°C by flunarizine dihydrochloride.
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posted on 2013-06-05, 00:30 authored by Annalisa Vitale, Natasha Thorne, Scott Lovell, Kevin P. Battaile, Xin Hu, Min Shen, Sabato D'Auria, Douglas S. AuldThe assay was performed with 260 nM PyAeADHII in 50 mM NaPO4 pH 7.5, 3 mM α-tetralone, 0.3 mM NADPH in the presence of 80–1200 µM flunarizine dihydrochloride in DMSO/isopropanol. a) Absorbance of NADPH (340 nm) was measured over time at different flunarizine concentrations. b) The rate of NADPH conversion (Δa.u. = absorbance unit change/minute) versus flunarizine concentration.
