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Inhibition of OCT1 activity by various BIM-related molecules.

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posted on 10.12.2015, 10:40 authored by Abdullah Mayati, Arnaud Bruyere, Amélie Moreau, Elodie Jouan, Claire Denizot, Yannick Parmentier, Olivier Fardel

HEK-OCT1 cells were incubated with 40 μM [14C]-TEA for 5 min at 37°C in the presence or absence of 50 μM verapamil or of various BIMs, used each at 5 μM. After washing with ice-cold PBS, intracellular accumulation of [14C]-TEA was determined by scintillation counting. Data are expressed as % of accumulation of TEA found in control cells, set at 100%, and are the means ± SEM of three independent experiments. *, p < 0.05 when compared with untreated cells (ANOVA followed by Dunnett's post-hoc test).