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Inhibition of NSC proliferation by PPARγ agonists.

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posted on 19.02.2013 by Saravanan Kanakasabai, Ecaterina Pestereva, Wanida Chearwae, Sushil K. Gupta, Saif Ansari, John J. Bright

NSCs dissociated from 7–10 day old neurospheres were cultured in 96 well tissue culture plates (1×104/0.2 ml/well) in NBM+B27 with different doses of EGF+bFGF (A) or 10 ng/ml EGF+bFGF in the presence of different doses of 15d-PGJ2 (B), ciglitazone (C) and ATRA (D). The cell proliferation/viability was measured by WST-1 assay. The values are means of triplicates±SD and the p values are expressed as *(p<0.05), **(p<0.01), and ***(p<0.001). The figure is a representative of three independent experiments.

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