Inhibition of GSK3β decreases protein levels and activity of HDAC6 in hippocampal neurons.

A. Western blot analysis of protein levels of HDAC6 in hippocampal neurons that were treated with LiCl (10 mM) or SB216763 (2 µM) for one hour. B. Quantification of Western blot shown in A (n = 3). C. HDAC6 activity assay of total protein lysate from hippocampal neurons that were treated with LiCl (10 mM) or SB216763 (2 µM) for one hour. D. HDAC6 activity assay of total protein lysate from hippocampal neurons that were treated with Akt inhibitor VIII (5 µM) for one hour. Efficiencies of the separation of cytoplasmic and nuclear phases in C and D are shown by Western blot using anti-GAPDH antibodies.