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Influence of the lcrF RNA thermometer on tissue colonization by Y. pseudotuberculosis.

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posted on 16.02.2012, 00:55 by Katja Böhme, Rebekka Steinmann, Jens Kortmann, Stephanie Seekircher, Ann Kathrin Heroven, Evelin Berger, Fabio Pisano, Tanja Thiermann, Hans Wolf-Watz, Franz Narberhaus, Petra Dersch

Strains YPIII (wildtype) and the yscW-lcrF variants YP90 (UU-28/-27CC) and YP95 (GUU-30/-28AAA) were infected intragastrically (5·108 CFU/mice) into BALB/c mice (n = 12/strain). After three days of infection, mice were sacrificed and the number of bacteria in homogenized host tissues and organs was determined by plating. Solid lines indicate the means. The statistical significances between the wildtype and the repressed and derepressed lcrF RNA thermometer variants were determined by Student's t test. P-values: *: <0.05; ***: <0.001.

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