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Infection of cells with live M. fermentans (M.f) did not alter the level of Topo I protein.

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posted on 27.08.2013 by Reuven Afriat, Shulamith Horowitz, Esther Priel

Samples from the MCF7 and U251 infected cells (described in Figure 1) were analyzed for detection of mycoplasmal DNA by PCR (A,B). Nuclear extracts derived from MCF7 and U-251 infected cells were assayed for Topo I protein level using Western blot analysis with anti-Topo I or anti-β-actin antibodies, and quantified by densitometric analysis using the EZquant software (C–F).