Induction of experimental dry eye.
Tear volumes (A) and fluorescein staining (B) in the eyes of C57BL/6 mice under experimental dry eye (EDE) conditions versus normal controls (NC). (A) EDE resulted in significant decreases in tear volume after 2 days. Tears were collected from the lateral canthus using cotton thread and reported as millimeters of wetted thread. Data are expressed as the mean +/− standard deviation per group from three independent experiments (≥3 mice per group for each experiment). * Denotes significance differences between treatment groups (determined with a parametric repeated measures ANOVA and Bonferroni post-test), p<0.001 in each instance). (B) Corneal integrity was assessed by fluorescein staining in EDE mice or normal controls after 5 or 10 days. Eyes were examined under blue light illumination at 20-x magnification. Photographs are representative of three independent experiments (≥3 mice per group for each experiment). Control mouse eyes are shown in the upper panels (a, b), EDE mouse eyes are shown in the lower panels (c, d). Arrows denote regions of fluorescein staining on the ocular surface.