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Increased responsiveness of SP-R210L(DN) cells to LPS.

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posted on 12.05.2015, 02:54 by Linlin Yang, Marykate Carrillo, Yuchieh M. Wu, Susan L. DiAngelo, Patricia Silveyra, Todd M. Umstead, E. Scott Halstead, Michael L. Davies, Sanmei Hu, Joanna Floros, Francis X. McCormack, Neil D. Christensen, Zissis C. Chroneos

Control and SPR210L(DN) cells were plated in 12-well tissue culture dishes at a density of 150,000 cells/well and cultured 24 hrs in RPMI/10% FBS. Cells were then treated with 100 ng/mL LPS for 2, 4, 8, 16, and 24 hrs. A) Intracellular staining of TNFα was performed in brefeldin A blocked cells 2 hrs after treatment with LPS. Dotted histograms show untreated cells. Black, red, and blue histograms show intracellular TNFα staining of control, SP-R210L(DN)1, and SP-R210L(DN)2 cells. B) The levels of secreted TNFα were measured by ELISA in media at indicated time points after treatment with LPS. (n = 6; ***p<0.005).