Increased expression of TRPV4 channels and recruitment of non-neuronal cells in 8-week-old amputation neuroma.
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Representative confocal images (60x with 2x scan zoom) of DAPI-stained cell nuclei ((A, D), blue) and TRPV4 channels ((B, E), gold) in uninjured tibial nerve tissue and within the neuroma bulb, respectively. Scale bar represents 15 μm. (G) Mean density of nuclei and TRPV4 channels (puncta) in uninjured tibial and neuroma tissues (n = 12). Density comparisons were made by 2 sample t-test (** p<0.01).