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Increased JAM-C expression has no influence on islet infiltration, viral clearance or antigen specific immune response.

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posted on 19.02.2013, 16:31 by Selina Christen, Ken Coppieters, Kerstin Rose, Martin Holdener, Monika Bayer, Josef M. Pfeilschifter, Edith Hintermann, Matthias G. von Herrath, Michel Aurrand-Lions, Beat A. Imhof, Urs Christen

- (A) Pancreas sections of RIP-LCMV-GP × pHHNS–JAM-C transgenic mice were stained with Hämatoxylin and the insulitis score was obtained from sections of 3 mice per group (number of islets analyzed per group are indicated). Scoring system: 0, no infiltration; 1, some peri-insular infiltration; 2, heavy peri-insular infiltration with some intra-insular infiltrates; 3, heavy intra-insular infiltration and/or islet scars. (B) Virus titers in the pancreas (P) and spleen (SP) on days 3, 7 and 14 after infection. Data are the mean ± SD of 3–4 mice per group. Det limit: Minimal number of plaques at highest tissue homogenate concentration required for statistical evaluation. (C) The presence of GP33-specific CD8+ T cells on day 10 after LCMV-infection in the spleen and PDLN of RIP-LCMV-GP or RIP-LCMV-GP × pHHNS-JAM-C mice was analyzed by intracellular cytokine staining (IFN-γ) after o/n in vitro stimulation with GP33 peptide. Data are the mean ± SD of six mice per group.

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