Increase in STAT3 phosphorylation in Müller cells after OSM treatment.
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Eyes were intravitreally injected with either PBS (2 µl) or OSM (3 µg in 2 µl), and retinas were harvested 1 hour later. Cryosections (16 µm) were stained for phosphorylated STAT3 (pSTAT3). Although no specific pSTAT3 staining was detected in the PBS treated control retina (A), a substantial increase in pSTAT3 immunoreactivity was seen in the OSM-treated retinas (B, C). The increased pSTAT3 is not co-localized with the RGC marker NeuN (B), but well co-localized with the Müller cell marker glutamine synthetase (C). RPE, retinal pigment epithelium; OS: photoreceptor outer segments; IS: photoreceptor inner segments; ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer. Scale bar: 50 µm.