Incorporation of selenium into mammalian SelO.
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Endogenous SelO was metabolically labeled with 75Se in HEK293T cells. Cell lysate was immunoprecipitated with anti-SelO antibody and visualized by (A) phosphorimaging and (B) Western blotting with anti-HA antibody. (C) Amido black staining shows equal loading. The 75Se labeled cell lysate was also immunoprecipitated with anti-HA antibody. SelO in this sample was visualized by (D) phosphorimaging and (E) Western blotting with anti-SelO antibody. (F) Amido black solution was used to stain the membrane for loading control.