In vivo evaluation of the BG34-10-Re-I/(AF488-MIF-siRNA) nanoparticles.

The nanoparticles loaded with the AF488-MIF siRNA were i.v. injected to Balb/c mice bearing day-10 4T1 tumor (∼ 5 x 7 mm). The i.v. injection was administered every other day for 7 days. A. Biodistribution of the AF488-MIF-siRNA at 2, 4 and 12 hours after the i.v. injection of the BG34-10-Re-I/(AF488-MIF-siRNA) nanoparticles. B. FACS analysis on the MIF protein expression in the F4/80 positive tumor-associated macrophages after the i.v. injection of the BG34-10-Re-I/(AF488-MIF-siRNA) nanoparticles. Mice treated with the nanoparticles loaded with the scrambled siRNA served as controls. Each group includes 8 mice and repeated twice.