In vivo effects of rmMFG-E8 in controlling TNF-α production during endotoxemia.
(A) WT mice were injected with rmMFG-E8 (0.4 µg/20 g BW) via i.p. for 2 h and then endotoxemia was generated by an overdose of i.p LPS (5 mg/kg BW) administration. Similarly, in another experiment, WT and MFG-E8−/− mice were treated with LPS (5 mg/kg BW) via i.p.. After 4 h, mice were euthanized, blood was collected and TNF-α measured by ELISA. (B, C) WT mice were treated with rmMFG-E8 (0.4 µg/20 g BW) for 2 h, followed by LPS (5 mg/kg BW) challenge. WT and MFG-E8−/− mice were injected with LPS (5 mg/kg BW) i.p.. After 2 h total RNA and after 4 h proteins were extracted from splenic tissues for TNF-α expression using real-time PCR and ELISA, respectively. All data are expressed as means ± SE (n = 3 independent experiments) and compared by one-way ANOVA and SNK method: *P<0.05 vs. sham control; #P<0.05 vs. endotoxemia and compared to rMFG-E8 treatment; †P<0.05 vs. endotoxemia and compared to MFG-E8−/−.