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In vitro virus suppression assay of TCR-transduced cell lines.

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posted on 2013-02-20, 15:29 authored by Eugene V. Barsov, Matthew T. Trivett, Jacob T. Minang, Haosi Sun, Claes Ohlen, David E. Ott

Flow cytometry analyses of mixed cultures consisting of effector CD8+ T-cell lines and a target autologous CD4+ T-cell clone that was untreated or exposed to either wild-type SIVmac239 or SIVmyr- are presented. Effectors are labeled above each column and targets are labeled at the left of each row. The effector CD8+ T cells in the co-cultures were stained with CellTrace Violet® and excluded from the analysis so that only the target cells were counted.