In vitro stress treatments change the pattern of MICA/B expression. A. Induction of TIA-1⁺ granules in Caco-2 cells.

Confocal microscopic analysis of Caco-2 cells treated during different periods of time with thapsigargin, sodium arsenite or fever-range temperature showing redistribution of TIA-1 (white) into stress granules. Nuclei (blue). (scan zoom 0,7, magnification 100×). B.- Redistribution of MICA/B in treated Caco-2 cells. Confocal microscopic analysis of Caco-2 cells treated during different periods of time with thapsigargin, sodium arsenite or fever-range temperature showing redistribution of MICA/B (red) in cytoplasmic aggregates. Nuclei (blue). (scan zoom 0,7, magnification 100×). C.- Distribution of MICA/B and TIA-1 in Caco-2 treated cells. Confocal microscopy of Caco-2 cells treated with thapsigargin (ER stress) or sodium arsenite (oxidative stress) for 1 hour, showing MICA/B (red) and TIA-1 (white) (magnification 100×). In both cases, MICA/B⁺ structures were not associated to stress TIA-1⁺ granules. (scan zoom 0,7, magnification 100×).