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In vitro osteogenesis accessed in three-month-old mice.

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posted on 21.02.2013, 00:45 by Wei Yao, Weiwei Dai, Mohammad Shahnazari, Aaron Pham, Zhiqiang Chen, Haiyan Chen, Min Guan, Nancy E. Lane

Bone marrow cells were obtained from WT female or male mice and cultured in osteogenic conditions for 14 days or 21 days and then stained for alkaline phosphatase (ALP, A and B, top pannels) or alizarin red (AR, A and B, lower pannels). The ALP level was quantified by absorbance at OD 410 nm (C)and normalized by total cell protein. The AR level was quantified by absorbance at OD 490 nm (D).

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