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In vitro multilineage differentiation of integration-free PB iPSCs.

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posted on 21.05.2013, 01:30 by Rui-Jun Su, David J. Baylink, Amanda Neises, Jason B. Kiroyan, Xianmei Meng, Kimberly J. Payne, Benjamin Tschudy-Seney, Yuyou Duan, Nancy Appleby, Mary Kearns-Jonker, Daila S. Gridley, Jun Wang, K-H. William Lau, Xiao-Bing Zhang

(A) Differentiation of PB iPSCs into MSCs. iPSC-MSCs show a typical MSC morphology and are capable of differentiation into adipocytes, osteoblasts and chondrocytes. Oil Red O stains the oil droplets of adipocytes. Alizarin Red stains the bone nodules formed by osteoblasts. Alcian Blue stains acid mucopolysaccharides synthesized and secreted by chondrocytes. (B) PB iPSC-derived hepatocytes show a typical morphology of hepatocytes at 25 days after hepatocytic differentiation. These cells also express markers AFP, albumin (ALB), and alpha 1-antitrypsin (α1-AT). The differentiated cells were stained with monoclonal antibody against AFP, goat anti-albumin, and goat anti-alpha 1-antitrypsin at 18 days after differentiation culture. (C) PB iPSC can be induced to differentiation into cardiomyocytes that express Troponin I marker. Cell nuclei were counterstained with DAPI.