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Imunohistochemical detection of different leukocyte populations in trout liver.

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posted on 2014-10-22, 03:20 authored by Rosario Castro, Beatriz Abós, Jaime Pignatelli, Louise von Gersdorff Jørgensen, Aitor González Granja, Kurt Buchmann, Carolina Tafalla

Representative photomicrographs of anti-IgM, anti-IgD, anti-IgT, anti-CD3, and anti-MHC-II positive staining in liver sections obtained from unstimulated fish (N = 4). Different phenotypes were observed among MHC-II+ cells including small lymphocyte-like round cells (arrows) and macrophage/dendritic-like cells (arrow heads). Some of these MHC-II+ cells appear as part of the endothelial layer of the blood vessels (asterisks). Counterstained with Mayer's haematoxylin. Scale bar represents 20 µm.