Important role for Chlamydia proteins in mediating actin coat formation.
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(A) HeLa cells expressing LifeAct-GFP and cytosolic DsRed were infected with C. trachomatis and at 44 hpi were treated for 4 h at 37°C with chloramphenicol, rifampicin, the type III secretion inhibitor C1, or left untreated. The numbers of inclusions with positive actin recruitment on their membranes were determined for each treatment. The effect of shorter treatments with chloramphenicol (B) or rifampicin (C) was determined by incubating infected cells with these compounds for times indicated. Error bars denote the SEM, >1000 inclusions were scored from a total of n = 3 experiments. ** p<0.001 *** p<0.0001.