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Impact on engraftment level of time delay between mice pre-conditioning and cell injections.

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posted on 17.09.2013, 01:31 by Jean Chevaleyre, Pascale Duchez, Laura Rodriguez, Marija Vlaski, Arnaud Villacreces, Véronique Conrad-Lapostolle, Vincent Praloran, Zoran Ivanovic, Philippe Brunet de la Grange

Female mice received 2 doses of busulfan (25mg/Kg each) at 24 hours, 4 days or 7 days intervals. Mice received 1000 non manipulated CD34+ cells by caudal injection and their bone marrow were analyzed 8 weeks later for A. total huCD45 chimerism, B. B lymphoid (▪) versus myeloid (□) cells ration in CD45+ cells, and C. huCFUs content per femur. Each mouse represented by (●), Median represented by (─).

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