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Impact of Ets and VEGFR-3 knockdown on mLECs in vitro.

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posted on 17.12.2012, 01:15 by Taeko Ichise, Nobuaki Yoshida, Hirotake Ichise

A. VEGFR-3 and Gapdh protein expression in Vegfr3-knockdown LECs. Upper panel, western blots; lower panel, quantitative analysis of western blots. Error bars represent the S.D.; n = 3. ****p<0.001 (vs. mLECs transfected with control siRNA). B. WST-1 assays using Vegfr3-knockdown mLECs (upper left panel) and Ets-knockdown mLECs (lower left panel), and BrdU assays using Vegfr3-knockdown mLECs (upper right panel) and Ets-knockdown mLECs (lower right panel). Error bars represent the S.D.; n = 12. *p<0.05, ****p<0.001 (vs. mLECs transfected with control siRNA in each assay). C. DiI-stained cellular networks of mLECs transfected with control, Ets1, Ets2, and Vegfr3 siRNAs on Matrigel. Scale bar = 500 µm. DiI-labeled areas were quantified and the mean area of DiI-labeled wild-type mLECs was normalized to 1. Error bars represent the S.D.; n = 3. **p<0.01, ***p<0.005, ****p<0.001 (vs. mLECs transfected with control siRNA).

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