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Immunosuppressive and complement-fixing effects of MAb DB81.

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posted on 19.07.2011, 01:37 by Samuele E. Burastero, Barbara Frigerio, Lucia Lopalco, Francesca Sironi, Daniela Breda, Renato Longhi, Gabriella Scarlatti, Silvana Canevari, Mariangela Figini, Paolo Lusso

A. Inhibition of PBMC proliferation by MAb DB81. PBMC from 4 healthy blood donors were incubated with the indicated stimuli in a 5-day proliferation assay. Either no inhibitors (“nil”) or the indicated mAbs (3 µg/ml) were added at the beginning of the culture time. Proliferation was measured by incorporation of tritiated thymidine in triplicate wells. The values indicate the mean ± standard error of the mean. B. Complement fixation by MAb DB81. Flow cytometry analysis of PM-1 cells treated with complement only (C, top left) or with C plus thymoglobulin (polyclonal rabbit antibodies to human thymocyte antigens, ATG, bottom right), C plus MAb Leu-3a (bottom left) or C plus DB81 (top right). Cells were stained with propidium iodide as a marker for cell death. Numbers in panels indicate the percent of stained (complement lysed, dead) cells, which fell within the double arrow markers in the different experimental settings.