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Immunolocalization of pectic homogalacturonan (HG) epitopes in epidermal cell walls.

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posted on 07.05.2014, 03:38 by Patricia M. Tilney, Abraham E. van Wyk, Chris F. van der Merwe

CLSM images of transverse sections of the pollen presenter epidermis. Double-headed arrows indicate position of bands of Igersheim. (A) Wax-embedded material immunolabelled with LM19 for de-esterified HG. This form of HG is mainly confined to those thickenings of Igersheim present in the outer tangential cell walls of the epidermal cells (for better resolution see C). Fluorescence of protoplasm in mesophyll cells due mainly to autofluorescence of chloroplasts. (B) Wax-embedded material immunolabelled with LM20 for methyl-esterified HG. This form of HG is abundantly present in primary cell walls as well as all the wall thickenings of Igersheim. (C) GMA-embedded material immunolabelled with LM19 and showing enlarged portion of distal end of epidermal cells. De-esterified HG mainly confined to outer portion (derived from primary cell wall?) of those thickenings of Igersheim present in the outer tangential cell walls of the epidermal cells, but also present in primary cell walls (middle lamella?) and outer boundary of bands of Igersheim (or electron-transparent zone between plasmalemma and wall thickenings—compare Figure 8), but essentially lacking in most of the bands themselves (bands marked with asterisks). (D) Wax-embedded material immunolabelled with LM20 and showing enlarged portion of distal end of epidermal cells. Methyl-esterified HG abundantly present in the bands of Igersheim, as well as throughout thickenings of the outer tangential cell walls.

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