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Immunohistochemistry for Bcl-2, Bcl-xL, survivin, p21 and cyclin D1.

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posted on 01.12.2014, 03:16 by Ying Zhang, Yaozhong Zhang, Hailong Yun, Raymond Lai, Min Su

By immunohistochemistry applied to formalin-fixed paraffin-embedded tissues, all proteins were detectable in most ESCC tumors. The staining was predominantly cytoplasmic for Bcl-2, Bcl-xL and surviving and nuclear for p21 and cyclin D1. Based on the staining intensity, tumors in our cohort were categorized into positive or negative (a) Cytoplasmic negative expression of Bcl-2 (b) Cytoplasmic positive expression of Bcl-2 (c) Cytoplasmic negative expression of Bcl-xL (d) Cytoplasmic positive expression of Bcl-xL (e) Cytoplasmic negative expression of survivin (f) Cytoplasmic positive expression of survivin (g) Nuclear negative staining of p21(h) Nuclear positive staining of p21 (i) Nuclear negative staining of cyclin D1 (j) Nuclear positive staining of cyclin D1(k) Cytoplasmic negative expression of STAT1(l) Cytoplasmic positive expression of STAT1 (IHC stain, scale bar, 20 µm).