Immunohistochemical staining of CD31(200×magnification) and quantification of microvessel density.
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(A). There were only a few microvessels in the subchondral bone of the necrotic femoral heads in the model group. (B). The prevention group showed increased microvessel density after EDHB treatment. (C). Bar graph shows the comparison between the model group and the prevention group. The microvessel density in the prevention group was significantly higher than that in the model group(P<0.05). The asterisk (*) shows statistically significant differences between the two groups. The arrows pointed to a lot of microvessel in the prevention group.