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Immunohistochemical staining and semi-quantitative analysis of HIF-1α and VEGF expression(200×magnification).

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posted on 22.09.2014 by Lihong Fan, Jia Li, Zefeng Yu, Xiaoqian Dang, Kunzheng Wang

HIF-1α and VEGF immunoreactivity were mainly observed in the osteoblasts and endothelial cells in both groups. (A). In the model group, weak immunoreactivity of HIF-1αwas found in the osteoblasts and endothelial cells. (B). After treatment with EDHB, the osteoblasts and endothelial cells showed a significantly high level of HIF-1αimmunoreactivity in the prevention group. (C) and (F). Bar graphs represent the mean optical density of HIF-1α and VEGF in bone marrow. Semi-quantitative analysis was based on at least 10 fields per section. The asterisk (*) shows statistically significant difference between the groups. (D). In the model group, weak immunoreactivity of VEGF was found in the osteoblasts and endothelial cells. (E). After treatment with EDHB, the osteoblasts and endothelial cells showed a significantly high level of VEGF immunoreactivity in the prevention group.

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