Immunohistochemical assay for KLF6 in placenta tissue section.
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Immunohistochemistry assays were performed as described in Material and Methods. The specific stain is shown as a brown precipitate and nuclei were blue counterstained with hematoxylin. Magnification is 600×. A. Stain pattern obtained for the commercial anti-KLF6 polyclonal antibody (Zf9, top) and for in house produced anti-KLF6 monoclonal antibody (clone 2C11, bottom) in term placenta tissue sections. B. Background control of the secondary detection complex EnVision® System Peroxidase Labeled Polymer (Dako, Carpinteira, USA). The placenta tissue section was incubated only with primary antibody dilution buffer, and the secondary detection complex mentioned above.