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Immunohistochemical analysis of mesothelin protein in lung tissue from FoxN null/Msln(+/+) and FoxN null/Msln(–/–) mice.

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posted on 13.08.2014, 03:06 by Jingli Zhang, Tapan K. Bera, Wenhai Liu, Xing Du, Christine Alewine, Raffit Hassan, Ira Pastan

A portion of lung tissues were fixed in 4% paraformaldehyde embedded in paraffin and sectioned 5 to 6 µm in thickness, then stained for MSLN. A–C: Lung tissue form FoxN null/Msln(+/+) stained with anti-Msln antibody (A: 100X; B: 200X magnification); or secondary antibody only (C: 100X magnification) as negative control. D–F: Lung tissue form FoxN null/Msln(–/–) stained with anti-Msln antibody (D: 100X; E: 200X magnification); or secondary antibody only (F: 100X magnification) as negative control. As expected strong mesothelin expression was detected in the mesothelial cell lining of the lung from FoxN null/Msln(+/+) mice but no expression in lung from FoxN null/Msln(–/–) mice.

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