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Immunohistochemical analysis of IL-17, IL-22, and IL-23 in human colon biopsies.

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posted on 06.09.2013, 01:50 by Savvas Petanidis, Doxakis Anestakis, Maria Argyraki, Margarita Hadzopoulou-Cladaras, Athanasios Salifoglou

Immunohistochemical staining of (A) IL-17, (B) IL-22, and (C) IL-23 in colon biopsies from patients with colorectal cancer. Paraffined sections of human colon biopsies were obtained from patients with colorectal cancer (b), and non-cancerous tissue (a). Human IL-17, IL-22, and IL-23, were detected using anti-human monoclonal antibodies from Santa Cruz. Staining with mouse IgG1 isotype was used as the negative control. Stained tumor cells are shown at a final magnification of ×400.