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Immunohistochemical analysis and quantification of CD4-positive T lymphocytes in human lung cancer.

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posted on 28.09.2015, 03:12 by G-Andre Banat, Aleksandra Tretyn, Soni Savai Pullamsetti, Jochen Wilhelm, Andreas Weigert, Catherine Olesch, Katharina Ebel, Thorsten Stiewe, Friedrich Grimminger, Werner Seeger, Ludger Fink, Rajkumar Savai

Human lung cancer tissue array was stained with CD4 antibody to detect T helper cells. (A) Quantification of CD4+ cells in lung cancer vs. healthy donor specimens. (B–E) Quantification of CD4+ cells based on (B) their pathology, (C) cancer stage, (D) tumor size, and (E) nodal status. Cell numbers are given as CD4-positive cells per 1000 cells. (F) Representative images of human lung sections stained with CD4 antibody based on their pathology. Scale bar = 25 μm.

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