Immunohistochemical analysis and quantification of CD4-positive T lymphocytes in human lung cancer.
Human lung cancer tissue array was stained with CD4 antibody to detect T helper cells. (A) Quantification of CD4+ cells in lung cancer vs. healthy donor specimens. (B–E) Quantification of CD4+ cells based on (B) their pathology, (C) cancer stage, (D) tumor size, and (E) nodal status. Cell numbers are given as CD4-positive cells per 1000 cells. (F) Representative images of human lung sections stained with CD4 antibody based on their pathology. Scale bar = 25 μm.