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Immunogold labeling of host proteins in purified influenza virions.

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posted on 2008-06-06, 02:37 authored by Megan L. Shaw, Kathryn L. Stone, Christopher M. Colangelo, Erol E. Gulcicek, Peter Palese

Influenza virions purified from the supernatant of infected Vero cells were immunogold labeled with antibodies against (A) Hemagglutinin, (B) CD9, (C) CD81 and (D) normal mouse IgG. Labeled virus was negatively stained with sodium silicotungstate and visualized by electron microscopy (50,000× magnification). The number of gold particles per virion is shown below (n = the number of virions counted).

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