Immunofluorescent localization of tubulin and KIFC1 during the middle stage of E. modestus spermiogenesis.
(A) DAPI nuclear staining. (B) Tubulin staining. Tubulin (green staining; arrows) localizes in sporadic areas within the lamellar complex (LCx). (C) KIFC1 staining. KIFC1 (red staining; arrows) localizes throughout the entire area of the LCx. (D) Phase contrast microscopy. This figure shows the general morphological appearance of the spermatid during the middle stage of spermiogenesis. (E) Merged immunofluorescent images. Tubulin (green staining; arrows) and KIFC1 (red staining; arrows) co-localize in relationship with the LCX. Nucleus (blue staining). (F) Diagram. This diagram shows the distribution of tubulin (green dots) and KIFC1 (red dots) in the spermatid during the middle stage of spermiogenesis. N: nucleus, LCx: lamellar complex, MT: microtubules, C: centriole.