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Immunofluorescent localization of GM130 (Golgi complex) during E. modestus spermiogenesis.

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posted on 30.09.2013 by Cong-Cong Hou, Wan-Xi Yang

(A–C) Early stage of spermiogenesis. (A) DAPI staining. (B) GM130 staining. GM130 (green staining; arrows) localizes in the cytoplasm near the periphery of the nucleus. (C) Merged immunofluorescent images. (D,E,F) Middle stage of spermiogenesis. (D) DAPI staining. (E) GM130 staining. GM130 (green staining; arrows) localizes near the LCx and along one side of the nucleus as the nucleus gradually deforms. (F) Merged immunofluorescent images. (G,H,I) Late stage of spermiogenesis. (G) DAPI staining. (H) GM130 staining. GM130 (green staining; arrows) localizes in the LCx and in the proacrosome near the AFS. (I) Merged immunofluorescent images. (J,K,L) Mature stage. (J) DAPI staining. (K) GM130 staining. GM130 (green staining; arrows) localizes in the acrosome. (L) Merged immunofluorescent images.

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